GSM5172709: SubAa272_rep3; Mus musculus; RNA-Seq - SRA

SRX10344546: GSM5172709: SubAa272_rep3; Mus musculus; RNA-Seq
1 ILLUMINA (HiSeq X Ten) run: 25.4M spots, 7.6G bases, 2.2Gb downloads

Submitted by: NCBI (GEO)

Study: Next Generation Sequencing Facilitates analysis of MODE-K SubAa272 and control cells genes expression

PRJNA714644 • SRP310712 • All experiments • All runs

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We performed the bulk RNA seq on the MODE-K SubAa272 and Control stable cell line and found the different genes expression in the setting of SubAa272 caused ER stress. Overall design: The RNA were extracted from 1x10^6 MODE-K SubAa272 and Control stable cells by RNeasy Plus Mini Kit(Qiagen,74134). Then Library preparation and transcriptome sequencing on an Illumina HiSeq X Ten platform were performed by Novogene Bioinformatics Technology Co., Ltd. to generate 150-bp paired-end reads.

Sample: SubAa272_rep3

SAMN18314016 • SRS8463662 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: HiSeq X Ten

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: 1x10^6 MODE-K SubAa272 and Control stable cells were harvasted and extracted by RNeasy Plus Mini Kit(Qiagen,74134). RNA libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM5172709

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 25.4M spots, 7.6G bases, 2.2Gb

Run	# of Spots	# of Bases	Size	Published
SRR13966623	25,380,462	7.6G	2.2Gb	2023-03-16

ID:: 13537498

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